Application of a new diagnostic technique for the detection of seed-borne pyrenophora species (leaf stripe and net bloch) on barley
Series: Home-Grown Cereals Authority Project Report ; No. 244Publication details: London : Home-Grown Cereals Authority (HGCA), 2001Subject(s): Cereal farming | Cereal farming, cereals marketing, grains | Barley diseasesDDC classification: 633.16 Summary: The aim of the project was to develop a diagnostic method for seed-borne Pyrenophora species on barley, based on DNA primers and the polymerase chain reaction (PCR) which was capable of delivering a rapid throughput routine test. The specific objectives were to optimise the PCR system and check the specificity of some of the primer sequences used, and finally to identify any practical problems which might arise in handling large numbers of tests.| Item type | Current library | Call number | Copy number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Book - Standard loan | CAFRE Greenmount Library | 633.16 THO (Browse shelf(Opens below)) | 1 | Available | 150560 |
The aim of the project was to develop a diagnostic method for seed-borne Pyrenophora species on barley, based on DNA primers and the polymerase chain reaction (PCR) which was capable of delivering a rapid throughput routine test. The specific objectives were to optimise the PCR system and check the specificity of some of the primer sequences used, and finally to identify any practical problems which might arise in handling large numbers of tests.
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